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Digital in line holography: 4-D imaging and tracking of microstructures and organisms in microfluidics and biology
J. Garcia Sucerquia, W. Xu, S.K. Jericho, M.H. Jericho, I. Tamblyn, H.J. Kreuzer ICO20: Biomedical Optics, Proc. SPIE 6026, 267-275 (2006) This work presents the application of digital in-line holographic microscopy (DIHM) as a powerful technique for four-dimensional imaging and tracking of microstructures and biological organisms. DIHM enables the reconstruction of three-dimensional positional information from a single hologram, allowing for the tracking of objects in both space and time. The technique is demonstrated for applications in microfluidics and biology, where the ability to monitor the trajectories and behavior of microscopic organisms and particles in three dimensions provides significant advantages over conventional two-dimensional microscopy. The method offers a lensless, low-cost approach to high-resolution volumetric imaging with broad potential for biological and microfluidic investigations. |
